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Synthesis of Polysaccharide‐Block‐Polypeptide Copolymer for Potential Co‐Delivery of Drug and Plasmid DNA
Author(s) -
Li Qianqian,
Liu Wenya,
Dai Jian,
Zhang Chao
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400454
Subject(s) - cationic polymerization , copolymer , chemistry , polysaccharide , dextran , drug delivery , gene delivery , polymer chemistry , nuclear chemistry , drug carrier , transfection , cytotoxicity , combinatorial chemistry , organic chemistry , in vitro , biochemistry , polymer , gene
A pH‐sensitive, biodegradable, and biocompatible polysaccharide‐block‐polypeptide Copolymer derivative {Ac‐Dex‐ b ‐PAsp(DET)} is synthetized from acetal‐modified dextran (Ac‐Dex) and diethylenetriamine (DET) grafted poly( L ‐aspartic acid) {PAsp(DET)} by using click and aminolysis reaction. The copolymer can self‐assemble into cationic nanopaticles for potential co‐delivery of plasmid DNA (pEGFP‐N3) and anticancer drug (doxorubicin, DOX), by using water/oil/water (w/o/w) emulsion method. Gel retardation assay reveals that pDNA can be effectively complexed into cationic nanoparticles at N/P ratio = 12. In vitro drug release behavior of DOX‐NPs and DOX/pDNA‐NPs is achieved by using fluorescence spectra and UV–Vis spectra and confocal laser scanning microscopy (CLSM). And, pEGFP‐N3‐NPs at N/P ratio = 42 presents the considerable potential in cell transfection. Cell viability assay shows that nanoparticles exhibit low cell cytotoxicity. These results suggest that the copolymer has excellent performance and potential for the co‐delivery of gene and drugs.