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Effects of Permeability and Living Space on Cell Fate and Neo‐Tissue Development in Hydrogel‐Based Scaffolds: A Study With Cartilaginous Model[Note a. aTBP1 is the abbreviation for TATA‐binding protein 1. ...]
Author(s) -
Fan Changjiang,
Wang DongAn
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400453
Subject(s) - self healing hydrogels , extracellular matrix , cell encapsulation , scaffold , chemistry , tissue engineering , hyaluronic acid , glycosaminoglycan , cell fate determination , regeneration (biology) , biophysics , microbiology and biotechnology , biomedical engineering , anatomy , biochemistry , biology , polymer chemistry , transcription factor , medicine , gene
One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior were evaluated, respectively. Three model hydrogel‐based constructs are fabricated by using photo‐crosslinkable hyaluronic acid as precursor and chondrocytes as model cell type. The better permeable hydrogels facilitate better cell viability and rapid proliferation, which lead to increased production of extracellular matrix (ECM), e.g. collagen, glycosaminoglycan. By prolonged culture, nano‐sized hydrogel networks inhibit neo‐tissue development, and the presence of macro‐porous living spaces significantly enhance ECM deposition via forming larger cell clusters and eventually induce formation of scaffold‐free neo‐tissue islets. The results of this work demonstrate that the manipulation and optimization of hydrogel microenvironments, namely permeability and living space, are crucial to direct cell fate and neo‐tissue formation.