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Amphiphilic PEO‐ b ‐PBLG Diblock and PBLG‐ b ‐PEO‐ b ‐PBLG Triblock Copolymer Based Nanoparticles: Doxorubicin Loading and In Vitro Evaluation
Author(s) -
Kakkar Dipti,
Mazzaferro Silvia,
Thevenot Julie,
Schatz Christophe,
Bhatt Anant,
Dwarakanath Bilikere S.,
Singh Harpal,
Mishra Anil K.,
Lecommandoux Sebastien
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400451
Subject(s) - copolymer , micelle , amphiphile , polymer chemistry , nanoparticle , materials science , doxorubicin , chemistry , chemical engineering , polymer , nanotechnology , organic chemistry , aqueous solution , surgery , medicine , chemotherapy , engineering
Huisgen's 1,3‐dipolar cycloaddition (“Click Chemestry”) has been used to prepare amphiphilic PEO‐b‐PBLG diblock and PBLG‐b‐PEO‐b‐PBLG triblock copolymers as potential carriers of anticancer drugs. Spherical and flower shaped micelles (D ≈100 nm) were obtained from diblock and triblock copolymers respectively. DOX was effectively encapsulated up to 18 wt.% and 50–60% of it was steadily released from the micelles over a period of 7 d. Flow cytometry and fluorescence microscopy confirmed the effective intracellular uptake as well as the sustained release of DOX from micelles. These results suggest that the diblock as well as triblock copolymers are promising carriers for intra‐cellular drug delivery.