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Phosphorylcholine‐Modified Chitosan Films as Effective Promoters of Cell Aggregation: Correlation Between the Films Properties and Cellular Response[Note a). a)Average of the values obtained from 1H NMR measurements ...]
Author(s) -
Qi Baowen,
Kujawa Piotr,
Toita Sayaka,
Beaune Grégory,
Winnik Françoise M.
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400439
Subject(s) - phosphorylcholine , chitosan , zeta potential , spheroid , dynamic light scattering , adhesive , chemistry , protein adsorption , polymer chemistry , polymer , chemical engineering , adsorption , quartz crystal microbalance , surface charge , materials science , analytical chemistry (journal) , nanotechnology , organic chemistry , biochemistry , layer (electronics) , nanoparticle , engineering , in vitro
This study describes chitosan‐phosphorylcholine (CH‐PC) films able to support the formation of cell aggregates (spheroids), which are important for tissue engineering and pharmacological studies. The surface topography, charge, thickness, and rheology of CH‐PC thin films were characterized by AFM, zeta‐potential measurements, SPR spectroscopy, and QCM‐D measurements. The CH‐PC films are highly hydrated gels, independently of the level of PC incorporation (15–40 mol‐% PC/glucosamine units). QCM‐D studies established that the amount of fibrinogen adsorbed on CH‐PC films decreased with increasing PC content. CH‐PC surfaces underwent a transition from moderately cell‐adhesive (CH‐PC15) to non‐adhesive (CH‐PC40). Optical micrographs of HUVEC and MCF‐7 cell lines cultured on CH‐PC surfaces showed that they form spheroids on CH‐PC25 and CH‐PC40 films.