Premium
Non‐Covalent Photo‐Patterning of Gelatin Matrices Using Caged Collagen Mimetic Peptides
Author(s) -
Li Yang,
San Boi Hoa,
Kessler Julian L.,
Kim Jin Hwan,
Xu Qingguo,
Hanes Justin,
Yu Seungju Michael
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400436
Subject(s) - gelatin , self healing hydrogels , chemistry , covalent bond , biophysics , methacrylate , polymer chemistry , biochemistry , polymer , organic chemistry , copolymer , biology
To address the downside of conventional photo‐patterning which can alter the chemical composition of protein scaffolds, we developed a non‐covalent photo‐patterning strategy for gelatin (denatured collagen) hydrogels that utilizes UV activated triple helical hybridization of caged collagen mimetic peptide (caged CMP). Here we present 2D and 3D photo‐patterning of gelatin hydrogels enabled by the caged CMP derivatives, as well as creation of concentration gradients of CMPs. CMP's specificity for binding to gelatin allows patterning of almost any synthetic or natural gelatin‐containing matrix, such as gelatin‐methacrylate hydrogels and corneal tissues. This is a radically new tool for immobilizing drugs to natural tissues and for functionalizing scaffolds for complex tissue formation.