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Novel Cationic Triblock Copolymer of Poly[2‐(dimethylamino)ethyl methacrylate]‐ block ‐poly(β‐amino ester)‐ block ‐poly[2‐(dimethylamino)ethyl methacrylate]: A Promising Non‐Viral Gene Delivery System
Author(s) -
Cordeiro Rosemeyre A.,
Farinha Dina,
Rocha Nuno,
Serra Arménio C.,
Faneca Henrique,
Coelho Jorge F. J.
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400424
Subject(s) - copolymer , cationic polymerization , methacrylate , polymer chemistry , biocompatibility , gene delivery , hela , chemistry , polymer , materials science , polymerization , organic chemistry , transfection , cell , biochemistry , gene
This manuscript reports the synthesis of a new cationic block copolymer based on poly[2‐(dimethylamino)ethyl methacrylate] and poly(β‐amino ester) from different polymerization strategies. For the first time, it is proposed a triblock copolymer based only on cationic segments, aiming a high biocompatibility, enhanced buffering capacity and stimuli‐responsive character in a single structure. The new block copolymer successfully condensed the plasmid DNA into nanosized polyplexes. The polyplexes were tested in two different cell lines revealing ∼4‐fold and ∼6‐fold (in HeLa cells), and ∼11‐fold (in COS‐7 cells) higher transgene expression than branched PEI and TurboFect™, respectively. These results show that this new block copolymer is a promising candidate to be used as a polymeric non‐viral vector.