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Improved Gene Transfection Efficacy and Cytocompatibility of Multifunctional Polyamidoamine‐Cross‐Linked Hyaluronan Particles
Author(s) -
Srivastava Akshay,
Cunningham Claire,
Pandit Abhay,
Wall J. Gerard
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400401
Subject(s) - transfection , chemistry , gene delivery , hyaluronic acid , cationic polymerization , biophysics , dna , cytotoxicity , amine gas treating , microbiology and biotechnology , gene , biochemistry , polymer chemistry , in vitro , biology , anatomy , organic chemistry
We describe a multi‐functional, cationic hyaluronic acid (HA)‐based gene carrier with improved transfection over non‐cross‐linked HA, and negligible cytotoxicity. Cationized particles are developed by cross‐linking HA chain carboxyl groups with polyamidoamine amine termini to produce well segregated particles of 350–400 nm with a surface charge density of +2 mV, compared with –35 mV for non‐cationized particles. A tethered antibody fragment retains ligand binding for cell targeting. Cationized and antibody‐linked particles complex plasmid DNA efficiently and the cationized particles successfully deliver reporter genes to bovine intervertebral disk cells as an intervertebral disk regeneration model.