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Adsorption of Fibronectin on Salt‐Etched Polyelectrolyte Multilayers and its Roles in Mediating the Adhesion and Migration of Vascular Smooth Muscle Cells
Author(s) -
Deng Jun,
Sun Mingcong,
Wang Shasha,
Han Lulu,
Mao Zhengwei,
Li Dan,
Chen Hong,
Gao Changyou
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400344
Subject(s) - fibronectin , adsorption , polyelectrolyte , adhesion , vascular smooth muscle , salt (chemistry) , chemistry , biophysics , cell adhesion , fibronectins , protein adsorption , chemical engineering , cell , smooth muscle , biochemistry , polymer , organic chemistry , biology , endocrinology , engineering
Abstract Protein adsorption on biomaterials strongly mediates the subsequent cell responses. Here adsorption of fibronectin (Fn) on salt‐treated PEI(PSS/PDDA) 7 multilayers was characterized. The amounts of adsorbed Fn increased linearly along with the increase of thickness of multilayers pretreated with 1 M and 5 M NaCl solutions (Multilayer‐1M and 5M), but was independent on the thickness of Multilayer‐3M. The normalized relative RGD activity of Fn were significantly higher on the Multilayer‐3M than on others. By comparison of cellular behaviors of VSMCs being cultured in the normal and Fn‐depleted serum‐containing medium, the significant role of Fn on modulating the behaviors of VSMCs was verified. The Fn adsorption model and its role on linking the biomaterials surface to the VSMCs behaviors are proposed.