Premium
A Cationic Poly(2‐oxazoline) with High In Vitro Transfection Efficiency Identified by a Library Approach
Author(s) -
Rinkenauer Alexandra C.,
Tauhardt Lutz,
Wendler Felix,
Kempe Kristian,
Gottschaldt Michael,
Traeger Anja,
Schubert Ulrich S.
Publication year - 2015
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400334
Subject(s) - cationic polymerization , cytotoxicity , chemistry , polymer , side chain , amine gas treating , transfection , polymer chemistry , oxazoline , organic chemistry , membrane , combinatorial chemistry , in vitro , biochemistry , catalysis , gene
To date, cationic polymers with high transfection efficiencies (TE) often have a high cytotoxicity. By screening an 18‐membered library of cationic 2‐oxazoline‐based polymers, a polymer with similar TE as linear poly(ethylene imine) but no detectable cytotoxicity at the investigated concentrations could be identified. The influence of the polymer side chain hydrophobicity and the type and content of amino groups on the pDNA condensation, the TE, the cytotoxicity, the cellular membrane interaction as well as the size, charge, and stability of the polyplexes was studied. Primary amines and an amine content of at least 40% were required for an efficient TE. While polymers with short side chains were non‐toxic up to an amine content of 40%, long hydrophobic side chains induced a high cytotoxicity.