Premium
Sulfated Hyaluronan Influences the Formation of Artificial Extracellular Matrices and the Adhesion of Osteogenic Cells
Author(s) -
Miron Alina,
Rother Sandra,
Huebner Linda,
Hempel Ute,
Käppler Iris,
Moeller Stephanie,
Schnabelrauch Matthias,
Scharnweber Dieter,
Hintze Vera
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400292
Subject(s) - fibrillogenesis , sulfation , chemistry , biophysics , extracellular matrix , glycosaminoglycan , chondroitin sulfate , ionic strength , adhesion , fibril , extracellular , cell adhesion , biochemistry , aqueous solution , organic chemistry , biology
The aim of this study is to compare differentially sulfated hyaluronan (sHA) derivatives and chondroitin sulfate (CS) with respect to their ability to influence the formation of artificial extracellular matrices (aECMs) during in vitro‐fibrillogenesis of collagen type I at high‐ and low‐ionic strength. Analysis is performed using turbidity, biochemical assays, atomic force (AFM), and transmission electron microscopy (TEM). In general, high‐sulfated glycosaminoglycans (GAGs) associate to a higher amount with collagen than the low‐sulfated ones. The addition of GAGs prior to fibrillogenesis at low‐ionic strength results in a dose‐dependent decrease in fibril diameter. At high‐ionic strength these effects are only obtained for the sHA derivatives but not for CS. Likewise, increasing concentrations and degree of GAG sulfation strongly affected the kinetics of fibrillogenesis. The impact of sulfation degree on F‐actin location and fiber formation in SaOS‐2 cells implies that adhesion‐related intracellular signaling is influenced to a variable extent.