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Monodisperse Carboxyl‐Functionalized Poly(Ethylene Glycol)‐Coated Magnetic Poly(Glycidyl Methacrylate) Microspheres: Application to the Immunocapture of β‐Amyloid Peptides
Author(s) -
Horák Daniel,
Hlídková Helena,
Hiraoui Mohamed,
Taverna Myriam,
Proks Vladimír,
Mázl Chánová Eliška,
Smadja Claire,
Kučerová Zdenka
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400249
Subject(s) - glycidyl methacrylate , chemistry , dispersity , ethylene glycol , methacrylate , capillary electrophoresis , polymer chemistry , peptide , chromatography , polymer , organic chemistry , polymerization , biochemistry
Identification and evaluation of small changes in β‐amyloid peptide (Aβ) levels in cerebrospinal fluid is of crucial importance for early detection of Alzheimer's disease. Microfluidic detection methods enable effective preconcentration of Aβ using magnetic microparticles coated with Aβ antibodies. Poly(glycidyl methacrylate) microspheres are coated with α‐amino‐ω‐methoxy‐PEG 5000 /α‐amino‐ω‐Boc‐NH‐PEG 5000 Boc groups deprotected and NH 2 succinylated to introduce carboxyl groups. Capillary electrophoresis with laser‐induced fluorescence detection confirms the efficient capture of Aβ 1–40 peptides on the microspheres with immobilized monoclonal anti‐Aβ 6E10. The capture specificity is confirmed by comparing Aβ 1–40 levels on the anti‐IgG‐immobilized particles used as a control.

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