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Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption
Author(s) -
Röttgermann Peter J. F.,
Hertrich Samira,
Berts Ida,
Albert Max,
Segerer Felix J.,
Moulin JeanFrançois,
Nickel Bert,
Rädler Joachim O.
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400246
Subject(s) - polyethylene glycol , peg ratio , adhesion , motility , fibronectin , copolymer , chemistry , protein adsorption , adsorption , biophysics , cell adhesion , polymer chemistry , chemical engineering , materials science , cell , polymer , biochemistry , organic chemistry , microbiology and biotechnology , biology , finance , engineering , economics
Adhesion and motility of cells on polyethylene glycol (PEG) engineered surfaces are of fundamental interest for the development of biotechnological devices. Here, the structure of PEG block copolymers physisorbed to surfaces by polyLlysine (PLL) or polypropylene oxide (PPO) is studied. Cell behavior on such surfaces incubated with fibronectin (FN) is analyzed via time‐lapse microscopy, the amount and the location of FN is determined via neutron reflectivity. While FN does not adsorb onto PPOPEG, 0.4–0.7 mg m −2 of FN is found in the vicinity of the PLL moiety of PLLPEG. Cells exhibit 21% increased motility on PLLPEG (5 kDa PEG chains) compared to pure FN layers, and 12% decreased motility for PLLPEG (2 kDa PEG chains). These findings suggest that by design of PEGylated surfaces cell migration can be controlled.