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Zwitterionic Red Fluorescent Polymeric Nanoparticles for Cell Imaging
Author(s) -
Li Haiyin,
Zhang Xiqi,
Zhang Xiaoyong,
Yang Bin,
Yang Yang,
Huang Zengfang,
Wei Yen
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400223
Subject(s) - biocompatibility , dynamic light scattering , fluorescence , copolymer , amphiphile , micelle , chemistry , fluorescence spectroscopy , polymerization , monomer , photochemistry , nanoparticle , chemical engineering , polymer , materials science , nanotechnology , organic chemistry , aqueous solution , physics , quantum mechanics , engineering
Phospholipid monomer and aggregation–induced emission (AIE) dye‐based dimers are incorporated via reversible addition‐fragmentation transfer polymerization to afford cross‐linked zwitterionic fluorescent copolymers. Such copolymers are prone to self‐assembly into fluorescent polymeric nanoparticles (FPNs) in physiological solution due to their amphiphilic nature. Characterization of these red FPNs by Fourier‐transform infrared spectroscopy and X‐ray photoelectron spectroscopy indicates the successful preparation of these zwitterionic copolymers. UV–visible absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy, and dynamic light scattering are performed to demonstrate the bright red fluorescence of the FPNs and their stable dispersibilities, even below the critical micelle concentration in physiological solution. Finally, studies of the biocompatibility and cell uptake behavior of the FPNs are conducted and show excellent biocompatibility for cell imaging application.