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Functionalized Polyglycerol Amine Nanogels as Nanocarriers for DNA
Author(s) -
Hellmund Markus,
Zhou Haixia,
Samsonova Olga,
Welker Pia,
Kissel Thomas,
Haag Rainer
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400144
Subject(s) - nanogel , nanocarriers , dynamic light scattering , amine gas treating , surface modification , chemistry , polymerization , hydrodynamic radius , copolymer , polymer chemistry , emulsion , chemical engineering , materials science , drug delivery , nanoparticle , polymer , nanotechnology , organic chemistry , engineering
Polyglycerol based nanogels (nPG) can function as cellular delivery systems. These nPGs are synthesized with different amine densities (nPG amines) by acid‐catalyzed epoxide‐opening polymerization using a mini‐emulsion approach and surface modification. All the synthesized nanogels are characterized by NMR, dynamic light scattering, and ζ‐potential, showing slightly positive surface charge and a homogeneous size of ≈100 nm. The use of these systems for delivery applications is demonstrated with regard to polyplex formation, cytotoxicity, and cellular uptake studies. It is depicted that the CE 50 value of the high loaded nPG amines is eight times higher than the low loaded ones. The influence of the amine loading percentage on the nanogel and the effects of polyvalency in these architecture is discussed.