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Bioengineered Silk Gene Delivery System for Nuclear Targeting
Author(s) -
Yigit Sezin,
Tokareva Olena,
Varone Antonio,
Georgakoudi Irene,
Kaplan D. L.
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400113
Subject(s) - lipofectamine , transfection , gene delivery , viability assay , chemistry , microbiology and biotechnology , biocompatible material , recombinant dna , cell culture , cell , biophysics , nanotechnology , biochemistry , biology , vector (molecular biology) , gene , materials science , biomedical engineering , medicine , genetics
Gene delivery research has gained momentum with the use of lipophilic vectors that mimic viral systems to increase transfection efficiency. Maintaining cell viability with these systems remains a major challenge. Therefore, biocompatible biopolymers that are designed by combining non‐immunological viral mimicking components with suitable carrier are explored to address these limitations. In the present study, dragline silk recombinant proteins are modified with DNA condensing units and the proton sponge endosomal escape pathway is utilized for enhanced delivery. Transfection efficiency in a COS‐7 cell line is enhanced compared to lipofectamine and polyethyleneimine (PEI), as is cell viability.