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Poly(2‐Hydroxyethyl Methacrylate)‐ b ‐Poly( L ‐Lysine) Cationic Hybrid Materials for Non‐Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts
Author(s) -
Johnson Renjith P.,
Uthaman Saji,
John Johnson V.,
Heo Min Seon,
Park In Kyu,
Suh Hongsuk,
Kim Il
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400071
Subject(s) - transfection , gene delivery , methacrylate , atom transfer radical polymerization , cytotoxicity , chemistry , copolymer , polylysine , biophysics , polymer chemistry , cationic polymerization , microbiology and biotechnology , polymer , biochemistry , in vitro , biology , gene , organic chemistry
In order to develop efficient and nontoxic gene delivery vectors, a series of biocompatible block copolymers, poly[(2‐hydroxyethyl methacrylate) 40 ‐ block ‐( L ‐lysine) n ] ( n = 40, 80, 120, 150), are prepared by combining an atom transfer radical polymerization of 2‐hydroxyethyl methacrylate with a ring‐opening polymerization of N ϵ ‐(carbobenzoxy)‐ L ‐lysine N ‐carboxyanhydride. The block copolymers are successfully condensed with plasmid DNA (pDNA) into nanosized (<200 nm) polyplexes. As a representative sample, p(HEMA) 40 ‐ b ‐p(lys) 150 is utilized to confirm the effective cellular and nuclear uptake of pDNA. The polymer/pDNA polyplexes exhibit very low cytotoxicity and enhanced transfection activity by being easily taken up into mouse embryonic fibroblast cell line (NIH 3T3). Thus, the chimeric block copolymers provide a means for developing versatile nonviral gene vectors harboring the ideal requirements of low cytotoxicity, good stability, and high transfection efficiency for gene therapy.