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Human Epidermal Keratinocyte Cell Response on Integrin‐Specific Artificial Extracellular Matrix Proteins
Author(s) -
Tjin Monica Suryana,
Chua Alvin Wen Choong,
Ma Dong Rui,
Lee Seng Teik,
Fong Eileen
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201400015
Subject(s) - extracellular matrix , fibronectin , integrin , microbiology and biotechnology , keratinocyte , laminin , basement membrane , chemistry , human skin , wound healing , cell adhesion , regeneration (biology) , cell , biology , immunology , biochemistry , in vitro , genetics
Cell‐matrix interactions play critical roles in regulating cellular behavior in wound repair and regeneration of the human skin. In particular, human skin keratinocytes express several key integrins such as alpha5beta1, alpha3beta1, and alpha2beta1 for binding to the extracellular matrix (ECM) present in the basement membrane in uninjured skin. To mimic these key integrin‐ECM interactions, artificial ECM (aECM) proteins containing functional domains derived from laminin 5, type IV collagen, fibronectin, and elastin are prepared. Human skin keratinocyte cell responses on the aECM proteins are specific to the cell‐binding domain present in each construct. Keratinocyte attachment to the aECM protein substrates is also mediated by specific integrin‐material interactions. In addition, the aECM proteins are able to support the proliferation of keratinocyte stem cells, demonstrating their promise for use in skin tissue engineering.