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Microsphere‐Based Intracellular Sensing of Caspase‐3/7 in Apoptotic Living Cells
Author(s) -
CárdenasMaestre Juan Manuel,
PérezLópez Ana M.,
Bradley Mark,
SánchezMartín Rosario M.
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300525
Subject(s) - flow cytometry , intracellular , apoptosis , caspase , microbiology and biotechnology , cytometry , chemistry , caspase 3 , biophysics , nanotechnology , biochemistry , biology , materials science , programmed cell death
A novel multifunctional probe to monitor intracellular enzymatic activity in living cells is successfully developed. Their use as accurate intracellular sensors by conjugation of an internal control (that gives an extra feature to both evaluate cellular‐uptake efficiency and track probes over time) is reported. In particular, a specific application of these multifunctional microspheres as sensors of caspase‐3/7 to monitor apoptosis by flow cytometry is described. The preparation of these devices together with a kinetic study towards caspase‐3 and caspase‐7 and their evaluation as flow cytometry probe in apoptotic living cells are reported.