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Dual Delivery of Vascular Endothelial Growth Factor and Hepatocyte Growth Factor Coacervate Displays Strong Angiogenic Effects
Author(s) -
Awada Hassan K.,
Johnson Noah R.,
Wang Yadong
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300486
Subject(s) - coacervate , hepatocyte growth factor , chemistry , vascular endothelial growth factor , growth factor , angiogenesis , endothelial stem cell , delivery system , microbiology and biotechnology , pharmacology , in vitro , cancer research , biochemistry , vegf receptors , medicine , biology , receptor
Controlled delivery of multiple growth factors (GFs) holds great potential for the clinical treatment of ischemic diseases and might be more therapeutically effective to reestablish vasculature than the provision of a single GF. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) are two potent angiogenic factors. However, due to rapid degradation and dilution in the body, their clinical potential will rely on an effective mode of delivery. A coacervate, composed of heparin and a biodegradable polycation, which protects GFs from proteolysis and potentiates their bioactivities, is developed. Here, the coacervate incorporates VEGF and HGF and sustains their release for at least three weeks. Their strong angiogenic effects on endothelial cell proliferation and tube formation in vitro are confirmed. Furthermore, it is demonstrated that coacervate‐based delivery of these factors has stronger effects than free application of both factors and to coacervate delivery of each GF separately.