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Additive Benefits of Chondroitin Sulfate and Oriented Tethered Epidermal Growth Factor for Vascular Smooth Muscle Cell Survival
Author(s) -
Lequoy Pauline,
Liberelle Benoît,
De Crescenzo Gregory,
Lerouge Sophie
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300443
Subject(s) - chondroitin sulfate , epidermal growth factor , chemistry , biophysics , vascular smooth muscle , adhesion , growth factor , microbiology and biotechnology , glycosaminoglycan , cell adhesion , cell growth , vascular endothelial growth factor , chondroitin , covalent bond , cell , biochemistry , biology , smooth muscle , endocrinology , cancer research , receptor , organic chemistry , vegf receptors
An anti‐apoptotic coating combining chondroitin sulfate (CS) and coiled–coil‐based tethering of epidermal growth factor (EGF) is designed for vascular applications. The oriented tethering strategy enables to reach higher EGF surface densities compared to the commonly used random covalent grafting, while using much lower concentrations of EGF during incubation. It also significantly improves vascular smooth muscle cell (VSMC) survival and resistance to apoptosis in serum‐free conditions. The comparison of CS and low‐fouling carboxymethylated dextran as a sublayer for growth factors highlights the tremendous benefit of CS thanks to its selective protein resistance and good cell adhesion properties. This approach can be tuned by capturing other growth factors on CS through coiled–coil interactions.