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In Situ Generation of Cell‐Laden Porous MMP‐Sensitive PEGDA Hydrogels by Gelatin Leaching
Author(s) -
Sokic Sonja,
Christenson Megan,
Larson Jeffery,
Papavasiliou Georgia
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300406
Subject(s) - gelatin , self healing hydrogels , porosity , leaching (pedology) , ethylene glycol , chemical engineering , peg ratio , tissue engineering , chemistry , materials science , polymer chemistry , biomedical engineering , composite material , organic chemistry , medicine , environmental science , finance , soil science , engineering , economics , soil water
Proteolytically degradable poly(ethylene) glycol (PEG) hydrogels have been investigated as tissue engineering scaffolds; however, cell invasion and tissue regeneration are limited by the rate of cell‐mediated degradation due to the small mesh size of the resultant crosslinked network. Gelatin leaching is combined with photopolymerization to form porous matrix‐metalloproteinase (MMP)‐sensitive PEG scaffolds under cytocompatible conditions in the presence of cells. Gelatin leaching allows control over pore size and porosity through selectivity of gelatin bead particle size and porogen loading, respectively. Increases in porogen loading lead to increased porosity, decreased compressive modulus and degradation time, and enhanced proliferation of encapsulated vascular smooth muscle cells.