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Gene Regulation by Intracellular Delivery and Photodegradation of Nanoparticles Containing Small Interfering RNA
Author(s) -
Murayama Shuhei,
Kos Petra,
Miyata Kanjiro,
Kataoka Kazunori,
Wagner Ernst,
Kato Masaru
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300393
Subject(s) - small interfering rna , gene silencing , rna interference , chemistry , intracellular , nanoparticle , gene expression , microbiology and biotechnology , transfection , rna , trans acting sirna , luciferase , gene , biophysics , biology , nanotechnology , biochemistry , materials science
Although the use of small interfering RNA (siRNA) is a promising technique for gene regulation, spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, a method for spatiotemporal regulation of genes with nanoparticles containing siRNA is reported. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3‐Luc cells, where the siRNA is released from the nanoparticles by UV irradiation for 30 s. The encapsulated siRNA only shows no gene‐silencing effects, but release of the siRNA upon UV radiation leads to sequence‐specific silencing of the luciferase gene in the cells. These results indicate that photodegradable siRNA‐containing nanoparticles can be useful for time‐ and space‐dependent regulation of gene expression in cells.