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The RGD Coupling Strategy Determines the Inflammatory Response of Human Primary Macrophages In Vitro and Angiogenesis In Vivo
Author(s) -
Bartneck Matthias,
Skazik Claudia,
Paul Nora E.,
Salber Jochen,
Klee Doris,
ZwadloKlarwasser Gabriele
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300362
Subject(s) - angiogenesis , in vivo , proinflammatory cytokine , chemistry , inflammation , immune system , macrophage , biophysics , in vitro , cytokine , materials science , microbiology and biotechnology , immunology , cancer research , biochemistry , biology
Surface modifications of implants are frequently done using bioactive peptides. However, immune cells such as macrophages might evoke a rejection of an implant due to an undesired activation by the materials. Here, the influence of different strategies for peptide immobilization onto (poly)‐vinylidene fluoride (PVDF) on inflammation and angiogenesis is studied. The inflammatory response of human primary macrophages is investigated by analyzing inflammatory cytokine expression. Surface roughness and adsorptive coupling have only minor effects on macrophage activation. Acrylic acid (AAc)‐based covalent RGD‐coupling leads to the most favorable cellular reaction, indicated by increased VEGF release. Chemical vapor deposition treated surfaces are inert, but additional covalent coupling of RGD induces a pronounced proinflammatory reaction. An in vivo angiogenesis study reveals that covalent coupling of RGD results in delayed but increased angiogenesis. It is concluded that for implant decoration with peptides, the substrate material has to be selected carefully to prevent inflammatory immune responses.