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p H ‐ and Redox‐ R esponsive Poly(ethylene glycol) and Cholesterol‐ C onjugated Poly(amido amine)s Based Micelles for Controlled Drug Delivery
Author(s) -
Cheng Weiren,
Kumar Jatin N.,
Zhang Yong,
Liu Ye
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300339
Subject(s) - micelle , chemistry , ethylene glycol , polymer chemistry , peg ratio , conjugated system , drug delivery , redox , polymerization , amine gas treating , nuclear chemistry , aqueous solution , organic chemistry , polymer , finance , economics
An optimized condition is identified to prepare linear poly(amido amine)s via Michael Addition polymerization of trifunctional amine, 4‐(aminomethyl)piperidine (AMPD), with an equimolar diacrylamide, N,N ‐cystaminebis(acrylamide) (BAC). Poly(ethylene glycol) (PEG) and cholesterol (CE) are conjugated to linear poly(BAC‐AMPD) through the reactions with the secondary amino groups in the backbone, respectively, to form poly(BAC‐AMPD)‐ g ‐PEG‐ g ‐CE. The chemical structures of poly(BAC‐AMPD) and poly(BAC‐AMPD)‐ g ‐PEG‐ g ‐CE are characterized using NMR and gel permeation chromatography (GPC). Transmission electron microscopy (TEM), dynamic light scattering (DLS) and 1 H NMR results show that micelles with PEG shells and hydrophobic cores composed of poly(BAC‐AMPD) and CE are formed via self‐assembly of poly(BAC‐AMPD)‐ g ‐PEG‐ g ‐CE in aqueous solution, and the micelles of poly(BAC‐AMPD)‐ g ‐PEG‐ g ‐CE can be degraded by the presence of L ‐dithiothreitol and show a limited cytotoxicity in vitro. The anti‐cancer drug, doxorubicin (DOX), can be loaded into the micelles. The DOX loaded micelles of poly(BAC‐AMPD)‐ g ‐PEG‐ g ‐CE show pH‐ and redox‐responsive drug release and redox‐induced formation of aggregates, and it is shown that the DOX loaded micelles can deliver DOX into cells and show a higher efficacy in killing cancer cells than free drug.