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Cholesterol Modification of ( B io) P olymers Using UV ‐ V is Traceable Chemistry in Aqueous Solutions
Author(s) -
Rasmussen Kasper F.,
Smith Anton A. A.,
RuizSanchis Pau,
Edlund Katrine,
Zelikin Alexander N.
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300286
Subject(s) - chemistry , vinyl alcohol , surface modification , polymer , reagent , chemical modification , aqueous solution , thiol , cyclodextrin , self healing hydrogels , bioconjugation , organic chemistry , polymer chemistry , combinatorial chemistry
Cholesterol modification of synthetic and biological polymers is achieved using of thiocholesterol (TC) and thiol‐disulfide exchange. TC is reacted with Ellman's reagent to produce a mixed disulfide (TC‐ER) which is activated towards thiol‐disulfide exchange. TC‐ER is used to obtain an inclusion complex with methyl‐β‐cyclodextrin, which is then employed to achieve cholesterol functionalization of a model peptide, synthetic polymers, and physical hydrogels based on poly(vinyl alcohol). It is anticipated that the established techniques will significantly broaden the use of cholesterol in bio‐ and nanotechnology and specifically biomedicine.