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Osteogenetic Properties of Electrospun Nanofibrous PCL Scaffolds Equipped With Chitosan‐ B ased Nanoreservoirs of Growth Factors
Author(s) -
Ferrand Alice,
Eap Sandy,
Richert Ludovic,
Lemoine Stéphanie,
Kalaskar Deepak,
DemoustierChampagne Sophie,
Atmani Hassan,
Mély Yves,
Fioretti Florence,
Schlatter Guy,
Kuhn Liisa,
Ladam Guy,
BenkiraneJessel Nadia
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300283
Subject(s) - chitosan , calvaria , osteopontin , nanofiber , biomineralization , tissue engineering , bone morphogenetic protein 2 , biomedical engineering , regenerative medicine , polycaprolactone , in vivo , chemistry , scaffold , biomaterial , regeneration (biology) , materials science , nanotechnology , in vitro , polymer , microbiology and biotechnology , chemical engineering , biochemistry , composite material , medicine , biology , engineering , cell
Bioactive implants intended for rapid, robust, and durable bone tissue regeneration are presented. The implants are based on nanofibrous 3D‐scaffolds of bioresorbable poly‐ϵ‐caprolactone mimicking the fibrillar architecture of bone matrix. Layer‐by‐layer nanoimmobilization of the growth factor BMP‐2 in association with chitosan (CHI) or poly‐ L ‐lysine over the nanofibers is described. The osteogenetic potential of the scaffolds coated with layers of CHI and BMP‐2 is demonstrated in vitro, and in vivo in mouse calvaria, through enhanced osteopontin gene expression and calcium phosphate biomineralization. The therapeutic strategy described here contributes to the field of regenerative medicine, as it proposes a route toward efficient repair of bone defects at reduced risk and cost level.