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Decoration of Electrospun Nanofibers with Monomeric Catechols to Facilitate Cell Adhesion
Author(s) -
Choi Ji Suk,
Messersmith Phillip B.,
Yoo Hyuk Sang
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300281
Subject(s) - catechol , nanofiber , ethylene glycol , chemistry , thiol , monomer , amine gas treating , polymer chemistry , adhesion , electrospinning , surface modification , cell adhesion , polymer , organic chemistry , chemical engineering , engineering
Monomeric catechols are displayed on the surface of polymeric nanofibers by robust catechol–thiol interactions to enhance cell adhesion and migration. Dihydroxyphenyl propionic acid is chemically conjugated to primary amine groups of poly(ϵ‐caprolactone)‐poly(ethylene glycol)‐amine (PCL‐PEG) nanofibers to display catechol moieties on the surface. At basic pH, catecholized nanofibers incorporate thiol groups at a five‐fold higher rate than at acidic pH, while catechol‐coated surfaces do not show any pH‐dependent binding. Live/dead cell staining indicates that the catecholized nanofibers do not exert any cytotoxic effects. Also, NIH 3T3 cells cultured on the catecholized nanofibers show increased attachment and migration that is proportional to the amount of the immobilized catechol moieties on the surface. These results clearly indicate that 6 nmol of monomeric catechols on the surface of nanofiber can promote cell adhesion and migration by thiol–catehol interactions.