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Enzymatic Synthesis of Amylose Brushes Revisited: Details from X‐ R ay Photoelectron Spectroscopy and Spectroscopic Ellipsometry
Author(s) -
Mazzocchetti Laura,
Tsoufis Theodoros,
Rudolf Petra,
Loos Katja
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300273
Subject(s) - x ray photoelectron spectroscopy , ellipsometry , chemistry , cystamine , monolayer , amylose , reductive amination , polymerization , self assembled monolayer , polymer chemistry , materials science , organic chemistry , chemical engineering , nanotechnology , polymer , thin film , biochemistry , starch , engineering , catalysis
The successful synthesis of amylose brushes via enzymatic “grafting from” polymerization and the detailed characterization of all synthetic steps by X‐ray photoelectron spectroscopy (XPS) and spectroscopic ellipsometry measurements are reported. Au and Si surfaces are amino‐functionalized with self‐assembled monolayers (SAMs) of cystamine and 3‐aminopropyldimethyethoxysilane (APDMES), respectively. Maltoheptaose is covalently attached to the amino‐functionalized Au and Si surfaces via reductive amination. Amylose brushes are grown from maltoheptaose modified Au and Si surfaces with enzymatic polymerization using potato phosphorylase and Rabbit Muscle phosphorylase, as evidenced by spectroscopic ellipsometry and XPS measurements.