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Chitosan‐ B ased Nanocoatings for Hypothermic Storage of Living Cells
Author(s) -
Bulwan Maria,
AntosiakIwańska Magdalena,
Godlewska Ewa,
Granicka Ludomira,
Zapotoczny Szczepan,
Nowakowska Maria
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300258
Subject(s) - chitosan , viability assay , chemistry , biophysics , bilayer , penetration (warfare) , nanotechnology , chemical engineering , membrane , cell , materials science , organic chemistry , biochemistry , biology , operations research , engineering
The formation of ultrathin chitosan‐based nanocoating on HL‐60 model cells and their protective function in hypothermic storage are presented. HL‐60 cells are encapsulated in ultrathin shells by adsorbing cationic and anionic chitosan derivatives in a stepwise, layer‐by‐layer, procedure carried out in an aqueous medium under mild conditions. The chitosan‐based films are also deposited on model lipid bilayer and the interactions are studied using ellipsometry and atomic force microscopy. The cells covered with the chitosan‐based films and stored at 4 °C for 24 h express viability comparable to that of the control sample incubated at 37 °C, while the unprotected cells stored under the same conditions do not show viability. It is shown that the chitosan‐based shell protects HL‐60 cells against damaging effect of hypothermic storage. Such nanocoatings provide protection, mechanical stability, and support the cell membrane, while ensuring penetration of small molecules such as nutrients/gases what is essential for cell viability.