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Enhanced Stem Cell Pluripotency in Surface‐Modified Electrospun Fibrous Matrices
Author(s) -
Zonca Michael R.,
Yune Philip S.,
Williams James K.,
Gu Minghao,
Unser Andrea M.,
Imbrogno Joseph,
Belfort Georges,
Xie Yubing
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300252
Subject(s) - methacrylamide , chemistry , stem cell , polymer chemistry , induced pluripotent stem cell , cell , tissue engineering , biophysics , embryonic stem cell , polymer , microbiology and biotechnology , biochemistry , organic chemistry , monomer , biomedical engineering , biology , acrylamide , gene , medicine
A previously screened “hit chemistry” ( N ‐[3‐(dimethylamino)propyl] methacrylamide) that supports strong attachment and long‐term self‐renewal of ES cells is selected and grafted to poly(ether sulfone) (PES) fibrous matrices through plasma‐induced graft polymerization. The 3D modified fibers exhibit higher cell proliferation and greater expression of pluripotency markers of mouse ES cells than 2D membranes. It is the first demonstration of scaling up an optimal synthetic surface chemistry in 2D using a high throughput synthesis, screening, and selection method to 3D that strongly influences pluripotent stem cell growth.