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Porous Hydrogels From Shark Skin Collagen Crosslinked Under Dense Carbon Dioxide Atmosphere
Author(s) -
FernandesSilva Susana,
MoreiraSilva Joana,
Silva Tiago H.,
PerezMartin Ricardo I.,
Sotelo Carmen G.,
Mano João F.,
Duarte Ana Rita C.,
Reis Rui L.
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300228
Subject(s) - self healing hydrogels , genipin , porosity , tissue engineering , carbon dioxide , supercritical carbon dioxide , chemical engineering , chemistry , chondrocyte , materials science , biomedical engineering , composite material , polymer chemistry , chitosan , in vitro , organic chemistry , medicine , biochemistry , engineering
The possibility to fabricate marine collagen porous structures crosslinked with genipin under high pressure carbon dioxide is investigated. Collagen from shark skin is used to prepare pre‐scaffolds by freeze‐drying. The poor stability of the structures and low mechanical properties require crosslinking of the structures. Under dense CO 2 atmosphere, crosslinking of collagen pre‐scaffolds is allowed for 16 h. Additionally, the hydrogels are foamed and the scaffolds obtained present a highly porous structure. In vitro cell culture tests performed with a chondrocyte‐like cell line show good cell adherence and proliferation, which is a strong indication of the potential of these scaffolds to be used in tissue cartilage tissue engineering.