Premium
Effective Tumor Treatment by VEGF si RNA Complexed with Hydrophobic Poly( A mino Acid)‐ M odified Polyethylenimine
Author(s) -
Chen Jie,
Tian Huayu,
Dong Xuan,
Guo Zhaopei,
Jiao Zixue,
Li Feifan,
Kano Arihiro,
Maruyama Atsushi,
Chen Xuesi
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300211
Subject(s) - polyethylenimine , gene silencing , chemistry , in vivo , in vitro , small interfering rna , amphiphile , luciferase , microbiology and biotechnology , biochemistry , rna , copolymer , cancer research , transfection , biology , gene , organic chemistry , polymer
Two copolymers are designed and synthesised for siRNA delivery based on polyethylenimine by grafting hydrophilic acrylamide segments and hydrophobic poly(γ‐benzyl L ‐glutamate). The amphiphilic PEI‐PBLG/siRNA complex demonstrates high gene silencing efficiency in the absence or presence of 10 vol% and 50 vol% sera in vitro. The anti‐tumor effects in vivo are evaluated in luciferase‐bearing mice expressing CT26 tumors. PEI‐PBLG/siVEGF complex provides a higher and more sustained suppressive effect by reducing VEGF mRNA expression in the tumors, leading to higher tumor growth inhibition efficacy. Further studies on the potential use of the PEI‐PBLG carrier system in mediating the silencing of genes other than VEGF or in other tumor models are recommended.