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Investigation of the Competition Between Cell/ S urface and Cell/ C ell Interactions During Neuronal Cell Culture on a Micro‐ E ngineered Surface
Author(s) -
Béduer Amélie,
GonzalesCalvo Inès,
Vieu Christophe,
Loubinoux Isabelle,
Vaysse Laurence
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300202
Subject(s) - neurite , cell , cell culture , microbiology and biotechnology , chemistry , cell growth , biophysics , nanotechnology , materials science , biology , biochemistry , genetics , in vitro
To investigate the respective roles of topography and cell/cell interactions in the development of a guided neuronal network on an engineered surface, micropatterned PDMS substrates were generated with different microgrooves geometry and investigated for the influence of cell density on neurite outgrowth and alignment. Through this systematic investigation, using a human neuronal stem cell line, the rules of neuronal network development and guiding could be learned. The results show that when cell density is increased the influence on neuritic outgrowth and alignment is very different for the various grooves geometries. The data emphasized the competition, in neurite development, between physical cues brought by surface topographical features and cell to cell communications. These results can be of particular interest for designing functional neuronal networks with a controlled architecture.