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Tailoring the Supramolecular Structure of Aminated Polyrotaxanes Toward Enhanced Cellular Internalization
Author(s) -
Yokoyama Nanako,
Seo JiHun,
Tamura Atsushi,
Sasaki Yoshihiro,
Yui Nobuhiko
Publication year - 2014
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300198
Subject(s) - internalization , supramolecular chemistry , chemistry , nanotechnology , biophysics , materials science , cell , biochemistry , molecule , organic chemistry , biology
The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated by flow cytometry and confocal laser scanning microscopy. AF‐545‐labeled aminated PRXs (APRXs) containing different numbers of threaded α‐cyclodextrins (CDs) and amino groups are synthesized; their cellular uptakes are analyzed using HeLa cells in serum. The APRX threaded CD number is discovered to be a more critical factor for enhancing cellular internalization than the APRX amine content. Additionally, APRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules. Because increased numbers of threaded CDs are related to increased PRX rigidity, the PRX rigid frame resulting from CD molecules threaded on a poly(ethylene glycol) (PEG) chain is suitable for intracellular tools in therapy and diagnosis.