Premium
Study of the Selective Uptake Progress of Aptamer‐ M odified PLGA Particles by Liver Cells
Author(s) -
Yu Dahai,
Zhang Yuying,
Mao Zhengwei,
Gao Changyou
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300165
Subject(s) - aptamer , endocytosis , plga , nucleolin , pinocytosis , chemistry , biophysics , bovine serum albumin , intracellular , cancer cell , conjugated system , microbiology and biotechnology , cell , biochemistry , cancer , biology , cytoplasm , in vitro , polymer , genetics , organic chemistry , nucleolus
It is of paramount importance to study the cellular uptake processes of particles with defined surface property, especially the uptake pathways and intracellular transportation. In this study, aptamer AS1411 molecules, which are known to specifically bind the over‐expressed nucleolin on cancer cell membrane, were conjugated onto bovine serum albumin‐decorated poly( D , L ‐lactide‐ co ‐glycolide; PLGA, Φ400 nm) particles with a density of 1–1.7 molecule/10 nm 2 . The aptamer‐modified PLGA particles were preferably ingested by liver cancer cells with higher amount and faster rate. The clathrin‐mediated endocytosis and macropinocytosis pathways played a more important role in uptake of the aptamer modified particles.