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Silk Fibroin/ G elatin Blend Films Crosslinked with Enzymes for Biomedical Applications
Author(s) -
Taddei Paola,
Chiono Valeria,
Anghileri Anna,
Vozzi Giovanni,
Freddi Giuliano,
Ciardelli Gianluca
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300156
Subject(s) - fibroin , gelatin , polymer chemistry , silk , chemistry , raman spectroscopy , thermal stability , tissue transglutaminase , macromolecule , polymer , adhesion , materials science , chemical engineering , enzyme , biochemistry , organic chemistry , composite material , physics , optics , engineering
Microbial transglutaminase (mTG) and mushroom tyrosinase (MT) are used to crosslink B. mori silk fibroin/gelatin (SF/G) films. Crosslinked and uncrosslinked SF/G films show no phase separation. The thermal behavior and the conformational structure of SF/G films are strongly affected by blending and enzymatic treatment. Formation of high thermally stable crosslinked macromolecular species is observed, suggesting the occurrence of strong intermolecular interactions between the two polymers as confirmed by FT‐Raman spectroscopy. Preliminary in vitro tests show that MT‐crosslinked blends with G amounts ≥40% and mTG‐crosslinked SF/G 60/40 films support C2C12 cardiomyocyte adhesion and proliferation.