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Protein Encapsulation in Biodegradable Polymeric Nanoparticles: Morphology, Fluorescence Behaviour and Stem Cell Uptake
Author(s) -
Rescignano Nicoletta,
Tarpani Luigi,
Tiribuzi Roberto,
Montesano Simona,
Martino Sabata,
Latterini Loredana,
Kenny Josè Maria,
Armentano Ilaria
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300140
Subject(s) - bovine serum albumin , plga , fluorescein isothiocyanate , chemistry , nanoparticle , fluorescence , biophysics , fluorescein , chemical engineering , emulsion , polymer chemistry , nanotechnology , chromatography , materials science , organic chemistry , physics , quantum mechanics , engineering , biology
The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC‐BSA) is reported. The protein is encapsulated in poly( D , L ‐lactide‐co‐glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady‐state and time‐resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer‐scale. Finally, FITC‐BSA‐loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.