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Photoenhanced Gene Transfection by a Star‐ S haped Polymer Consisting of a Porphyrin Core and Poly( L ‐lysine) Dendron Arms
Author(s) -
Ma Dong,
Zhao Yi,
Zhou XiaoYan,
Lin QianMing,
Zhang Yi,
Lin JianTao,
Xue Wei
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300139
Subject(s) - porphyrin , dendrimer , hela , transfection , chemistry , polymer , gene delivery , polylysine , in vitro , gene transfer , polymer chemistry , cytotoxicity , lysine , microbiology and biotechnology , photochemistry , gene , organic chemistry , biochemistry , biology , amino acid
A star‐shaped polymer (PP‐PLLD) consisting of a porphyrin (PP) core and poly( L ‐lysine) dendron arms (PLLD) is synthesized by the click reaction, and its ability to deliver pEGFP is investigated in this paper. It is found that PP‐PLLD has a good buffer capacity and can form compact complexes with pEGFP. In vitro assay indicates that PP‐PLLD shows photoenhanced gene transfection efficiency. PP‐PLLD consisting of only third generation PLLD shows a higher transfected cell number than PEI under a Xe lamp at the N/P ratio of 20, and meanwhile shows a neglectable cytotoxicity to HeLa cells. Therefore, PP‐PLLD with suited irradiation is a promising nontoxic and photoinducible effective gene delivery strategy, which should be encouraged in gene therapy.