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Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine‐ and Biotin‐Tagged Biomolecules into Cells
Author(s) -
Jana Batakrishna,
Mondal Goutam,
Biswas Atanu,
Chakraborty Indrani,
Saha Abhijit,
Kurkute Prashant,
Ghosh Surajit
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300129
Subject(s) - biomolecule , biotin , avidin , surface modification , graphene , chemistry , fluorescence , nanotechnology , streptavidin , bioconjugation , combinatorial chemistry , biochemistry , materials science , physics , quantum mechanics
A versatile method of dual chemical functionalization of graphene oxide (GO) with Tris‐[nitrilotris(acetic acid)] (Tris‐NTA) and biotin for cellular delivery of oligohistidine‐ and biotin‐tagged biomolecules is reported. Orthogonally functionalized GO surfaces with Tris‐NTA and biotin to obtain a dual‐functionalized GO (DFGO) are prepared and characterized by various spectroscopic and microscopic techniques. Fluorescence microscopic images reveal that DFGO surfaces are capable of binding oligohistidine‐tagged biomolecules/proteins and avidin/biotin‐tagged biomolecules/proteins orthogonally. The DFGO nanoparticles are non‐cytotoxic in nature and can deliver oligohistidine‐ and biotin‐tagged biomolecules simultaneously into the cell.