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Glycol Chitosan‐ B ased Nanogel as a Potential Targetable Carrier for siRNA
Author(s) -
Pereira Paula,
Morgado Daniela,
Crepet Agnès,
David Laurent,
Gama Francisco M.
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300123
Subject(s) - nanogel , ethylene glycol , chemistry , hela , biophysics , micelle , chitosan , viability assay , peg ratio , polymer chemistry , cell , drug delivery , biochemistry , organic chemistry , biology , finance , aqueous solution , economics
A self‐assembled glycol chitosan nanogel (GC) is synthesized by chemically grafting hydrophobic chains onto a polysaccharide, which is comprehensively characterized. The obtained macromolecular micelle is decorated with folate‐conjugated poly(ethylene glycol) (PEG) (GCFA). An average size distribution of 250 and 200 nm is observed, respectively for the GC and GCFA nanogels. Differential cell localization is observed on incubating the materials with HeLa cells. Whereas the GC nanogel is detected on the cell surface, GCFA is localized in the cytoplasm. The cell viability is not compromised by the nanogels. Interestingly, GC nanogel is poorly internalized by bone marrow derived macrophages (BMDMs), and GCFA is not phagocytosed. Given its ability to complex siRNA, the targetable GC nanogel can be a promising vehicle for siRNA delivery.