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Hyaluronic Acid Nanohydrogels as a Useful Tool for BSAO Immobilization in the Treatment of Melanoma Cancer Cells
Author(s) -
Montanari Elita,
Capece Sabrina,
Di Meo Chiara,
Meringolo Martina,
Coviello Tommasina,
Agostinelli Enzo,
Matricardi Pietro
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300114
Subject(s) - hyaluronic acid , chemistry , biocompatible material , conjugate , hydrogen peroxide , cytotoxicity , covalent bond , aldehyde , melanoma , biochemistry , in vitro , combinatorial chemistry , cancer research , biomedical engineering , organic chemistry , medicine , mathematical analysis , mathematics , anatomy , catalysis
An alternative anticancer therapy based on the use of bovine serum amine oxidase (BSAO), an enzyme that converts polyamines over‐expressed in malignant cells, into hydrogen peroxide and aldehyde(s), thus inducing high cytotoxicity in cancer cells, was recently proposed. With the aim of improving the system efficacy by exploiting a nanotechnology approach, BSAO is covalently immobilized onto injectable nanohydrogels (NHs) based on cholesterol‐graft‐hyaluronic acid (HA‐CH), a biocompatible conjugate that spontaneously leads to self‐assembled structures in aqueous solutions. In this study, the physicochemical properties of the HA‐CH‐based NHs and the NHs cytocompatibility are reported. The properties of the NHs‐BSAO system are also studied in terms of protein residual activity, both in vitro and on a model melanoma cell line.