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Nanobody‐ F unctionalized Polymersomes for Tumor‐ V essel Targeting
Author(s) -
Debets Marjoke F.,
Leenders William P. J.,
Verrijp Kiek,
Zonjee Marleen,
Meeuwissen Silvie A.,
OtteHöller Irene,
van Hest Jan C. M.
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300039
Subject(s) - polymersome , chemistry , azide , biophysics , liposome , peptide , transmembrane protein , fusion protein , small molecule , combinatorial chemistry , biochemistry , biology , recombinant dna , polymer , amphiphile , receptor , organic chemistry , gene , copolymer
Targeted carrier systems (e.g., liposomes or nanoparticles) are used to specifically deliver drugs to a site of interest. Site‐direction can be achieved by attachment of targeting molecules, such as peptides, DNA/RNA, or antibodies, to the surface of the carrier. Here, the formation of polymersomes with tumor‐targeting potential is described. A single‐domain antibody (A12) that specifically targets PlexinD1 (a transmembrane protein overexpressed in tumor vasculature) is equipped with an azide‐functionality using expressed protein ligation. This azide‐containing A12 can subsequently be attached to BCN‐functionalized polymersomes using a strain‐promoted azide alkyne cycloaddition, thereby forming polymersomes with tumor‐targeting potential.