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Caveolae‐ M ediated Endocytosis of Conjugated Polymer Nanoparticles
Author(s) -
Lee Junghan,
Twomey Megan,
Machado Christian,
Gomez Giselle,
Doshi Mona,
Gesquiere Andre J.,
Moon Joong Ho
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300030
Subject(s) - endocytosis , caveolae , chemistry , pinocytosis , conjugated system , nanotechnology , microbiology and biotechnology , biophysics , biochemistry , polymer , signal transduction , materials science , biology , cell , organic chemistry
Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae‐mediated endocytosis (CvME). CvME is further confirmed by high co‐localization with caveolin‐1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non‐toxicity, and non‐destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery.