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Phenotype‐ I ndependent Effects of Retroviral Transduction in Human Dental Pulp Stem Cells
Author(s) -
Egbuniwe Obi,
Grant Andrew D.,
Renton Tara,
Silvio Lucy Di
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300020
Subject(s) - dental pulp stem cells , phenotype , microbiology and biotechnology , telomerase reverse transcriptase , stem cell , senescence , transfection , cell culture , biology , pulp (tooth) , gene , telomerase , genetics , pathology , medicine
An immortalized human dental pulp stem cell (DPSC) line of an odontoblastic phenotype is established to circumvent the normal programmed senescence and to maintain the cell line's usefulness as a tool for further study of cellular activity. DPSCs are isolated from human dental pulp tissues and transfected using hTERT. The influence of this process on the DPSC phenotype and the mRNA expression of oncogenes involved in cellular senescence is investigated. The results reveal an absence of altered DPSC morphology and phenotype following the exogenous introduction of the hTERT gene, which is coupled with a significant reduction in p16 mRNA expression. This provides insight into how to circumvent in vitro dental pulp stem cell death following the exogenous introduction of hTERT.