Premium
Poloxamine–Cyclodextrin–Simvastatin Supramolecular Systems Promote Osteoblast Differentiation of Mesenchymal Stem Cells
Author(s) -
Simões Susana M. N.,
Veiga Francisco,
TorresLabandeira Juan J.,
Ribeiro Ana Cristina F.,
Concheiro Angel,
AlvarezLorenzo Carmen
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201300017
Subject(s) - simvastatin , supramolecular chemistry , chemistry , cyclodextrin , alkaline phosphatase , osteoblast , scaffold , mesenchymal stem cell , in vitro , biophysics , biochemistry , biomedical engineering , microbiology and biotechnology , organic chemistry , enzyme , molecule , pharmacology , biology , medicine
Osteogenic/osteoinductive systems combine simvastatin, poloxamine Tetronic 908 (T908) and α‐cyclodextrins (αCDs) in a supramolecular network that enhances the solubility/stability of the simvastatin hydroxy acid form and synergistically promotes osteoblast differentiation. Incorporation of 5% αCD transforms dilute T908 solutions (as low as 2% copolymer) into gels, enhances the osteoinductive activity of T908, and provides simvastatin sustained release for more than one week, which results in higher and more prolonged alkaline phosphatase (ALP) activity. The performance of the intrinsically osteoinductive polypseudorotaxane scaffold can be easily tuned by modifying the concentrations of T908, αCD, and simvastatin in a certain range of values. Moreover, the use of affordable, stable materials that can be sterilized applying a conventional method make the supramolecular gels advantageous candidates as scaffolds to be applied in the critical defect using minimally invasive techniques.