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Macromol. Biosci. 2/2012
Author(s) -
Hiraishi Tomohiro,
Yamashita Koichi,
Sakono Masafumi,
Nakanishi Jun,
Tan LiuTzea,
Sudesh Kumar,
Abe Hideki,
Maeda Mizuo
Publication year - 2012
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201290005
Subject(s) - enzyme , monomer , biocatalysis , catalysis , chemistry , cover (algebra) , replication (statistics) , cell , biochemistry , stereochemistry , biology , organic chemistry , polymer , reaction mechanism , mechanical engineering , virology , engineering
Cover: Cell surface display of enzymes as whole‐cell catalysts may be a better choice in comparison with typical enzyme catalysts because of advantages like autonomous replication, preventing the toxicity of the expressed proteins, the stabilization of the displayed enzymes due to self‐immobilization on the cells, and so on. The present manuscript focuses on functionally active expression of poly[( R )‐3‐hydroxybutyrate] (PHB) depolymerase on the cell surface of E. coli and its potential use as a biocatalyst for ( R )‐3‐hydroxybutyrate (3HB) monomer production from insoluble PHB. Further details can be found in the article by T. Hiraishi,* K. Yamashita, M. Sakono, J. Nakanishi, L.‐T. Tan, K. Sudesh, H. Abe, and M. Maeda* on page 218 .