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In Vitro Neo‐Cartilage Formation on a Three‐ D imensional Composite Polymeric Cryogel Matrix
Author(s) -
Bhat Sumrita,
Lidgren Lars,
Kumar Ashok
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200484
Subject(s) - cartilage , agarose , osteoarthritis , matrix (chemical analysis) , biomedical engineering , composite number , materials science , gelatin , chemistry , composite material , anatomy , biology , pathology , medicine , chromatography , biochemistry , alternative medicine
Limited blood supply and the avascular nature of articular cartilage restricts its self repair capacity, frequently leading to osteoarthritis. This work focuses on scaffolds for tissue repair from natural polymers, for example gelatin, chitosan, and agarose in the form of composite. A novel way of fabrication, known as cryogelation, is presented, in which matrices are synthesized at sub‐zero temperature. Cell seeded scaffolds incubated under appropriate conditions result in the accumulation of matrix components on the surface of the gel in the form of neo‐cartilage. Neo‐cartilage exhibits similarity to native cartilage with respect to its physical, mechanical and biochemical properties. Based on the similarities of neo‐cartilage to the native cartilage, it can provide a new approach for the treatment of localised joint injuries.