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A Generic Micropatterning Platform to Direct Human Mesenchymal Stem Cells from Different Origins Towards Myogenic Differentiation
Author(s) -
Yu Ting,
Chua Chee Kai,
Tay Chor Yong,
Wen Feng,
Yu Haiyang,
Chan Jerry K. Y.,
Chong Mark S. K.,
Leong David Tai,
Tan Lay Poh
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200481
Subject(s) - myogenesis , mesenchymal stem cell , micropatterning , microbiology and biotechnology , bone marrow , downregulation and upregulation , stem cell , cellular differentiation , myosin , chemistry , biology , myocyte , immunology , biochemistry , materials science , nanotechnology , gene
Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone‐marrow‐derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs isolated from three different sources (bone marrow, fetal tissue, and adipose) is reported. All the well‐aligned cells, regardless of source, predominantly commit to myogenic lineage, as shown by the significant upregulation of myogenic gene markers and positive myosin heavy chain staining. It is demonstrated that our novel micropattern can be used as a generic platform for inducing myogenesis of MSCs from different sources and may also have the potential to be extended to induce other lineage commitment.