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Microwell Chips for Selection of Bio‐macromolecules that Increase the Differentiation Capacities of Mesenchymal Stem Cells
Author(s) -
Hsu Shanhui,
Ni YuHui,
Lee YungChun
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200472
Subject(s) - mesenchymal stem cell , spheroid , macromolecule , chemistry , microbiology and biotechnology , adhesion , stem cell , cell adhesion , cell , cadherin , nanotechnology , biophysics , materials science , biology , biochemistry , in vitro , organic chemistry
Microwell chips (25 mm × 25 mm) are fabricated to select proper substrates for growing three‐dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio‐macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (≈10 5 ) are needed for each chip. The expression level of N‐cadherin, a cell‐adhesion molecule, is used as an indicator for cell‐cell interactions. MSC spheroids expressing the highest N‐cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio‐macromolecules that enhance the differentiation potential of MSCs.