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In Vitro Evaluation of Combined Sulfated Silk Fibroin Scaffolds for Vascular Cell Growth
Author(s) -
Liu Haifeng,
Ding Xili,
Bi Yanxue,
Gong Xianghui,
Li Xiaoming,
Zhou Gang,
Fan Yubo
Publication year - 2013
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/mabi.201200470
Subject(s) - fibroin , scaffold , chemistry , in vitro , silk , cell adhesion , adhesion , microbiology and biotechnology , thrombin , tissue engineering , biomedical engineering , biophysics , materials science , platelet , biochemistry , immunology , biology , composite material , medicine , organic chemistry
A combined sulfated silk fibroin scaffold is fabricated by modifying a knitted silk scaffold with sulfated silk fibroin sponges. In vitro hemocompatibility evaluation reveals that the combined sulfated silk fibroin scaffolds reduce platelet adhesion and activation, and prolong the activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). The response of porcine endothelial cells (ECs) and smooth muscle cells (SMCs) on the scaffolds is studied to evaluate the cytocompatibility of the scaffolds. Vascular cells are seeded on the scaffolds and cultured for 2 weeks. The scaffolds demonstrate enhanced EC adhesion, proliferation, and maintenance of cellular functions. Moreover, the scaffolds inhibit SMC proliferation and induce expression of contractile SMC marker genes.